The non-treated cells used as the negative control showed no change in cell viability. In the presence of the Au NPs, rapid death of the HepG2 liver cancer cells in vitro was observed after the application of 13 MHz RF for 8 min. Distilled water ( 95%, by conventional cell viability assay procedures. HAuCl4⋅3H2O, sodium citrate tribasic dehydrate, and other chemicals were purchased from Sigma-Aldrich and used without purification. Specific response of the Au NPs resulted in an increase in temperature sufficient to induce thermal cell death in vitro. We tested our Au NPs in a mediated electric-hyperthermia system using high RF (13 MHz) in a hepatocellular carcinoma cell line (HepG2). Existing methods of Au NP preparation use seed nanoparticles, with a conducted seed-growth tactic reaction after Au ion addition, to increase the Au NP size under dilute conditions (few nM).7 Furthermore, this environment-friendly method allows for control of the Au NP size and potential one-pot scale-up by increasing Au NP concentrations up to ~10 nM. We now report the preparation of Au NPs using an electro-reduction method without toxic chemicals (such as strong reducing agents). reported that Au NP colloids generate heat in excess of 50 ☌ when subjected to RF radiation, which suggests their potential use in RF ablation to induce selective tumor cell death.5 However, toxic chemicals such as strong reducing agents are often used in the synthesis of Au NPs and are often difficult to remove this is a potential drawback in the use of Au NPs.6 Ideally the NPs should be responsive to specific RFs, which do not induce electric vibration of metal ions present in healthy cells, and naturally possess biocompatibility. Therefore, due to insufficient ablation, tumor recurrence often occurs, whereas excessive ablation may result in collateral damage to normal tissues and the adjacent organs.4 Recently, several research groups have en-deavored to overcome these problems by using a combi-nation of RF ablation and nanoparticles (NPs). Although the RF electrode is positioned into the target tumor, the range of heating cannot be controlled, and thermal damage occurs in both tumor cells and healthy cells sur-rounding the electrode. However, RF ablation is a relatively non-specific treat-ment. To induce cellular death via coagu-lation necrosis, the ideal temperature to be achieved during RF ablation ranges from 50 to 60 ☌.3 This process creates a localized area of heating through electric vibration of metal ions in the tumor cells. Radiofrequency (RF) ablation is currently the most popular and widely used thermal therapy for the treatment of various solid tumors.1,2 In this treatment, a needle-type electrode is inserted directly into the targeted tumor to deliver an RF (375–500 kHz) alternating current.
#Dls malvern zetasizer supporting information pdf
Copy Citation PDF KSCI KPUBS JATS XML HTML (Department of Radiology, Seoul National University Bundang Hospital, College of Medicine) In comparison with batch measurements of DLS using the Zetasizer Nano alone, the online coupling considerably increases the resolving power of DLS, extending the range of applications for which it can provide highly detailed information.(Laboratory of Nano-bio Materials Chemistry, Department of Applied Bioscience, College of Life Science, CHA University) Using Malvern’s Zetasizer Nano and Postnova’s FFF systems together, provides information not accessible by other means, and is highly complementary to Malvern’s Viscotek gel permeation chromatography / size exclusion chromatography (GPC/SEC) product range.įield-Flow Fractionation enables the separation of nanoparticles and large macromolecules, which cannot be analyzed by GPC/SEC, and the addition of the Zetasizer Nano as an online DLS detector allows the subsequent measurement of absolute particle size. Malvern Instruments and Postnova Analytics have announced that they are co-operating to offer combined FFF (Field-Flow Fractionation) and DLS (Dynamic Light Scattering) solutions.
![dls malvern zetasizer supporting information dls malvern zetasizer supporting information](https://i.ebayimg.com/images/g/Tb4AAOSwtmBfvqyF/s-l64.jpg)
The combination of FFF and DLS creates an unique High Resolution Particle Sizing Solution